Developed for Experts
Cytofit is developed in close collaboration with leading CyTOF experts within the Norwegian cancer research cluster.
How It Works
Simple workflow to streamline biomarker discovery in CyTOF data.
Platform Basics
Projects
Organise datasets by project and iterate efficiently in different branches while keeping results structured and easy to review.
Inputs
Use standard FCS files for CyTOF data together with metadata to define patients, cohorts, time points, and comparison groups.
Outputs
Generate fully transparent and shareable results with feature tables, figures, and a summary report that documents all steps.
Features
Cytofit is an all-in-one CyTOF platform that streamlines the entire process, from uploaded files to biomarker insights.
Data Cleaning
Standard preprocessing steps to make datasets analysis-ready and comparable, with clear, repeatable transformations that improve consistency across samples and support reliable downstream analysis, including gating, clustering, and statistical comparisons.
Calibration
Calibration uses beads to normalise data within each sample and across samples, while removing bead signals automatically. It takes just a few clicks instead of sample-by-sample adjustments, making the workflow easier and faster.
Phenotyping
Identify biologically relevant subpopulations using hierarchical clustering, and score events against phenotypes in a customisable cell lineage for cell type abundance and biomarker expression plots.
Statistical Comparisons
Define comparison groups directly from metadata (for example patient, cohort, time point, or treatment arm). This is often binary, but you can combine additional metadata columns to run multi-level comparisons and summarise biomarker readouts across multiple groups.
Reporting
Generate clear summaries of final results with feature tables and key figures, plus a structured report that documents samples, metadata, the exact data cleaning + gating parameters used, and statistical results with run details.
Transparent - No Black Box
Cytofit makes it easy to review your analysis workflows: how groups were defined, which biomarkers were compared, and how data cleaning + gating were carried out. All steps in the workflow are documented with gate types and parameters, making results fully transparent and reproducible.