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The all-in-one CyTOF Platform

Upload mass cytometry FCS files, clean data, phenotype it and analyse abundances
— all in a single cloud platform.

Built to simplify mass cytometry workflows. ​

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Input

  • Project Details

  • FCS Files

  • Sample Metadata

  • Comparison Labels

Cytofit steps

  • Filtering

  • Data Cleaning

  • Calibration

  • ​Phenotyping

Outputs

  • Biomarker Insights

  • Cell Population Plots​

  • Statistical Tests

  • Summary Report

Core Features

Cytofit covers the full CyTOF analysis workflow, from data cleaning and gating to clustering, comparisons, and reporting.

Data Cleaning

Turn raw FCS files into analysis-ready data with consistent and repeatable preprocessing.

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Calibration

Automatic bead detection and calibration within and across samples, making the process easier and more transparent.

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Phenotyping

Identify absolute and relative cell populations and statistical insights using automatic phenotyping algorithms. 

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Statistical Comparisons

Define cohorts from metadata and compare cell type distributions across binary or multi-group study designs. 

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Reporting

Export figures, tables, and a shareable report that documents inputs, parameters, and results.

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Transparent Workflows

Review every decision and parameter so results are explainable, reproducible, and easy to share.

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Assisted Data Cleaning

Run algorithm-guided data cleaning across biomarkers in one run, or fine-tune detectors where needed. Get consistent results across samples without turning automatic workflows into a black box.

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The Hematopoietic Lineage

Build and refine your custom hematopoietic lineage visually, for faster phenotyping and comparisons.​​​

Dataset Visualisation

Create cohorts from metadata and compare cell abundances across binary or multi-group study designs with clear, publication-ready outputs.​​

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Developed for Experts

Cytofit is developed in close collaboration with leading CyTOF experts within the Norwegian cancer research cluster.

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"Cytofit has revolutionized our analytical workflow, enabling us to focus on the science rather than the process. Its clarity and speed make it indispensable for complex studies like OxyTarget"

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Sebastian Meltzer

Resident Oncologist and Postdoc, AUH

Common Applications

CyTOF is a high-dimensional single-cell method that uses metal-tagged antibodies and time-of-flight mass spectrometry to measure many markers per cell. It is typically used for: 

High-Dimensional Immune Profiling
Biomarker Discovery & Stratification
Longitudinal Immune Dynamics
Clinical Immune Monitoring
Host Response to Infection & Vaccination
Immune Landscape Mapping

Practical CyTOF tips, new analysis features, and Cytofit product updates.

Made for CyTOF

Working with mass cytometry data?
Learn how Cytofit simplifies your analysis workflow end-to-end.

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